Smurf Assay for Zebrafish Larvae

Smurf assays were conducted according to Dambroise et al. [47 (link)] by placing zebrafish larvae in 50 mL Falcon tubes containing 2.5% blue #1 (erioglaucine disodium salt, Sigma) for 30 min at room temperature. They were then rinsed under aquarium water until no more blue coloration could be found in the eluate, anesthetized in 0.016% tricaine (MS-222; 3-amino benzoic acid ethyl ester, Sigma) and imaged on a bright-field stereomicroscope (Leica MZ125) equipped with a Leica DFC295 digital camera. After imaging, DNA was extracted for genotyping analyses.

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Raby L., Völkel P., Hasanpour S., Cicero J., Toillon R.A., Adriaenssens E., Van Seuningen I., Le Bourhis X, & Angrand P.O. (2021). Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish. Cells, 10(11), 3142.

Publication 2021

MS-222; 3-amino benzoic acid ethyl ester MZ125 DFC295 digital camera

Amino acid Assays Benzoic Camera Erioglaucine Ester Larvae Ms 222 Salt Tricaine Zebrafish

Corresponding Organization : Inserm

Other organizations : University of Tehran, Université d'Artois

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Variable analysis

independent variables

Placing zebrafish larvae in 50 mL Falcon tubes containing 2.5% blue #1 (erioglaucine disodium salt, Sigma) for 30 min at room temperature

dependent variables

Presence of blue coloration in the eluate
Phenotype of zebrafish larvae observed during imaging

control variables

Rinsing of zebrafish larvae under aquarium water until no more blue coloration could be found in the eluate
Anesthetizing zebrafish larvae in 0.016% tricaine (MS-222; 3-amino benzoic acid ethyl ester, Sigma)
Imaging zebrafish larvae on a bright-field stereomicroscope (Leica MZ125) equipped with a Leica DFC295 digital camera

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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