Leishmania spp. and Microfilariae Detection

Positive PCR products of the 5.8S-ITS2-28S rRNA of microfilariae and two PCR products of hsp70C of Leishmania spp. from dog were confirmed by Sanger sequencing (Macrogen, Korea). Reads were compared with those reported in the database GenBank using the BLAST tool. Obtained sequences were assembled with Geneious Prime software version 2022.0.1 [31 ]. Revised sequences of microfilarial species were reported to GenBank with accession numbers MZ473246, MZ473247, MZ473248, MZ473249, MZ468150, MZ468151, and MZ474199. In the same way, detected sequences from L. infantum were reported with the accession numbers MZ605427 and MZ605428.

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Pérez-Ramírez R.D., Lugo-Vargas R., Petano-Duque J.M., Cruz-Méndez J.S, & Rondón-Barragán I.S. (2023). First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia. Veterinary World, 16(1), 94-103.

Publication 2023

Sanger sequencing

28s rrna Leishmania Microfilarial

Corresponding Organization :

Other organizations : University of Tolima

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Variable analysis

independent variables

PCR products of the 5.8S-ITS2-28S rRNA of microfilariae
PCR products of hsp70C of Leishmania spp. from dog

dependent variables

Sequences of microfilarial species
Sequences of L. infantum

control variables

Control variables not explicitly mentioned

controls

Positive controls: Sanger sequencing (Macrogen, Korea)
Negative controls: Not mentioned

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