Structural Modeling of Fibulin2-Notch2 Interaction

Sequence search-based modeling of Fibulin2 was performed using the SWISS-MODEL database (swissmodel.expasy.org) based on the structure of Dll1 (PDB ID: 4xbm.2. A, 31.44% shared identity). The SWISS-MODEL-predicted structures were analyzed for the QMEAN Z score, which included the cumulative Z score of the Cβ, all atoms, solvation and torsion values. The Notch2 domain structure was downloaded from the RCSB PDB database (www1.rcsb.org). The initial docking between the Fibulin2 and Notch2 structures was performed with Z-Dock (3.0.2). Then, model screening was conducted using PDBePISA to identify all conformations (www.ebi.ac.uk/msd-srv/ssm/).

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Li S.D., Xing W., Wang S.C., Li Y.B., Jiang H., Zheng H.X., Li X.M., Yang J., Guo D.B., Xie X.Y., Jiang R.Q., Fan C., Li L., Xu X, & Fei J. (2023). Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing. Experimental & Molecular Medicine, 55(2), 443-456.

Publication 2023

Dock Notch2

Corresponding Organization :

Other organizations : Daping Hospital, Army Medical University, Affiliated Hospital of Southwest Medical University, Montreal Neurological Institute and Hospital, Southwest Jiaotong University

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Variable analysis

independent variables

Sequence search-based modeling of Fibulin2 using the SWISS-MODEL database based on the structure of Dll1 (PDB ID: 4xbm.2. A, 31.44% shared identity)

dependent variables

QMEAN Z score, which included the cumulative Z score of the Cβ, all atoms, solvation and torsion values
Conformations identified using PDBePISA

control variables

Notch2 domain structure downloaded from the RCSB PDB database

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