HEK293T cells were transfected with miRNA mimics and inhibitors as we have previously described [14 (link)]. Briefly, cells were seeded in 6-well cell culture plates (BD Biosciences) and transfected 24h later. For experiments with miRNA mimics, the cells were transfected with a combination of synthetic miRNA mimics for hsa-miR-92a1–5p (50nM), hsa-miR-27b-5p (50nM) and hsa-miR-1260a (25nM) or 125 nM scrambled sequence control MISSION® microRNA mimics (Sigma-Aldrich; St. Louis, MO). For experiments with miRNA inhibitors, the cells were transfected with pEZX-AM03 plasmid-based miRNA inhibitors from Genecopoeia (Rockville, MD) against hsa-miR-92a-1–5p (cat# HmiR-AN0831-AM03-B), hsa-miR-27b-5p (cat # HmiR-AN0360-AM03-B) and hsa-miR-1260a (cat# HmiR-AN0100-AM03-B) or a control plasmid with scrambled sequence (cat# CmiR-AN0001-AM03-B). All transfections were performed using lipofectamine 2000 (Thermo Fisher). Cells were recovered at 37°C for 24h, then incubated for 24h at 32°C, 37°C, or 39.5°C, lysed in RIPA, and immunoblotted for TCF7, ß-catenin, and ß-tubulin.