Biogenic Amine Analysis in Baechu Kimchi

BA analyses in Baechu kimchi samples and assay menstrua were conducted according to protocols described in a previous study [34 (link)]. For an internal standard, 1,7-Diaminoheptane (1 mg/mL; Sigma) was used. Tyramine hydrochloride, histamine dihydrochloride, cadaverine dihydrochloride, putrescine dihydrochloride, spermine tetrahydrochloride, spermidine trihydrochloride, tryptamine, and β-phenylethylamine hydrochloride were used to prepare standard solutions (all from Sigma). The analysis procedures included extraction of BAs, preparation of BA standard solutions, derivatization of BAs, and chromatographic separation of BAs. Both limits of detection and limits of quantification for all BAs were about 0.10 µg/mL in standard solutions and 0.01–0.10 mg/kg and 0.02–0.31 mg/kg, respectively, in food matrices [47 (link)].