Bioluminescence Imaging of Saa3 Promoter-luc Mice

In vivo bioluminescence imaging was performed as previously described [12 (link),13 (link)]. Briefly, before acquiring images, 150 mg/kg body weight D-luciferin (Promega, Madison, WI, USA) was administered intraperitoneally to all Saa3 promoter-luc mice in both models five minutes after substrate administration using the NightOWL II Imaging Systems LB983 (Berthold Technologies, Bad Wildbad, Germany). For each mouse, the signal intensity was quantified as the sum of all detected photon counts per second and was presented as counts/s (cps). All images were adjusted to the same scale as the minimum and maximum luminescent intensities for any given analysis.