Cyclotron-Produced Zirconium-89 Purification

⁸⁹Zr was produced at Memorial Sloan Kettering Cancer Center on a TR19/9 cyclotron (Ebco Industries Inc.) via the ⁸⁹Y(p,n)⁸⁹Zr reaction and purified to yield ⁸⁹Zr with a specific activity of 5.28–13.43 mCi/μg (470–1195 Ci/mmol) of zirconium.^{40 (link)} Activity measurements were performed using a CRC-15R Dose Calibrator (Capintec). For the quantification of activities, experimental samples were counted on an Automatic Wizard^{2 (link)}γ-Counter (PerkinElmer). All in vivo experiments were performed according to protocols approved by the Memorial Sloan Kettering Institutional Animal Care and Use Committee. A purity of greater than 95% was confirmed using radio-TLC for all of the ⁸⁹Zr-labeled cRGDY-PEG-C′ dots.

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Chen F., Ma K., Zhang L., Madajewski B., Zanzonico P., Sequeira S., Gonen M., Wiesner U, & Bradbury M.S. (2017). Target-or-Clear Zirconium-89 Labeled Silica Nanoparticles for Enhanced Cancer-Directed Uptake in Melanoma: A Comparison of Radiolabeling Strategies. Chemistry of materials : a publication of the American Chemical Society, 29(19), 8269-8281.

Publication 2017

CRC-15R Dose Calibrator Automatic Wizard2 (link)γ-Counter

Cancer Cyclotron Ebco Institutional animal care and use committee Zirconium

Corresponding Organization :

Other organizations : Kettering University, Cornell University

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Variable analysis

independent variables

Production of ⁸⁹Zr at Memorial Sloan Kettering Cancer Center on a TR19/9 cyclotron (Ebco Industries Inc.) via the ⁸⁹Y(p,n)⁸⁹Zr reaction

dependent variables

Specific activity of ⁸⁹Zr (5.28–13.43 mCi/μg or 470–1195 Ci/mmol of zirconium)
Purity of ⁸⁹Zr-labeled cRGDY-PEG-C′ dots (greater than 95% confirmed using radio-TLC)

control variables

Activity measurements performed using a CRC-15R Dose Calibrator (Capintec)
Quantification of activities using an Automatic Wizard² γ-Counter (PerkinElmer)
All in vivo experiments performed according to protocols approved by the Memorial Sloan Kettering Institutional Animal Care and Use Committee

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