Immunohistochemistry of paraffin-embedded formalin-fixed slides of tumor was performed using anti-Gli1 antibody (1:1000, Cat# 66905–1-Ig, Proteintech, USA) or anti-Ki65 antibody (1:1000, GB11499, Servicebio, China). After rinsing with PBS, the sections were incubated with the secondary antibody HRP (1:200, G23303, Servicebio, China) for 50 min. The slides were dehydrated and then sealed, after chromogenic detection by adding 3,3’-Diaminobenzidine. The images were acquired using Olympus I × 71 (Olympus, Japan). The immunohistochemical staining score was graded according to the German semi-quantitative scoring system52 (link).