Reconstruction of Metabolic Network for E. coli
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Corresponding Organization :
Other organizations : La Jolla Bioengineering Institute, University of California, San Diego, Northwestern University, McCormick (United States), SRI International, École Polytechnique Fédérale de Lausanne
Protocol cited in 54 other protocols
Variable analysis
- Additional reactions added to the metabolic network for iJR904
- Reactions removed from the metabolic network
- Gene to protein to reaction (GPR) assignments
- The metabolic network for iJR904 as the starting point
- Biochemical characterization studies of E. coli-specific reactions
- E. coli genome annotation as a citation source for biochemical characterization studies
- Metabolite charge determined using pKa values at pH 7.2
- Reactions designated as enzymatically catalyzed or spontaneous, elementally and charge balanced, and either reversible or irreversible
- Positive control: Comparison of the content of iJR904 with the EcoCyc database
- Negative control: Not specified
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