Genetic Mouse Models for Epigenetic Regulation

Wnt1-Cre2 (Jackson Laboratory, 022137)(Lewis et al., 2013 (link)), Mesp1-Cre (Saga et al., 1999 (link)), R26R-LacZ (Soriano, 1999 (link)), and Hdac2^Flox (Anokye-Danso et al., 2011 (link)) mice have been previously described. Frozen embryos of Hdac1^{tm1a(EUCOMM)Wtsi} (strain EM:04097) were obtained from the European Mouse Mutant Archive (EMMA). The University of Massachusetts Medical School Transgenic Animal Facility regenerated cryopreserved embryos. The knockout-first allele, Hdac1^{tm1a(EUCOMM)Wtsi}, mice were bred with wild type mice and are annotated Hdac1^LacZ/+. Hdac1^{tm1a(EUCOMM)Wtsi} (Hdac1^LacZ) mice were bed with ACTB-FLPe (Jackson Laboratory, 003800) to generate Hdac1^Flox mice. Hdac1^Flox mice were bred with Hdac2^Flox, R26R-LacZ, and Wnt1-Cre2 mice to generate mice for analysis. All animal protocols were approved by the University of Massachusetts Medical School Institutional Animal Care and Use Committee (IACUC).