Cells were washed with ice-cold phosphate-buffered saline (PBS) buffer (Invitrogen; 20012050) and lysed with harvest buffer (10 mM 4-[2-hydroxyethyl]-1-piperazineethanesulfonic acid [HEPES; pH 7.9], 50 mM sodium chloride [NaCl], 500 mM sucrose, 0.1 mM ethylenediaminetetraacetic acid [EDTA], and 0.5% Triton X-100) supplemented with 1% Halt inhibitors (Thermo Fisher Scientific; 78443) for 10 min. The cell lysate was centrifuged at 3000 rpm for 3 min at 4 °C, and the supernatant containing cytosolic proteins was transferred to a new tube. The pellet containing nuclear proteins was dissolved with nuclear lysis buffer (10 mM HEPES [pH 7.9], 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM egtazic acid, and 0.1% NP40) supplemented with 1% Halt inhibitors (Thermo Fisher Scientific; 78443) for 5 min at 4°C. Then, the solution was sonicated at 4°C for six cycles (1 cycle = 30 s sonication [high intensity] and 30 s cooldown) using a Bioruptor Plus sonication (Diagenode; UCD-300). After sonication, the solution was centrifuged at 15,000 rpm for 10 min, and the supernatant was retained for nuclear extraction. Protein concentration was determined with the BCA assay (Thermo Fisher Scientific, 23227). Immunoblotting was performed as previously described (4 (link)). All the blots are representative of at least two independent experiments.