Intravitreal Injection of AAV or CTB-FITC

AAV vectors (5μl, titers at 1.0 x 10¹²) or cholera toxin β subunit conjugated fluorescein isothiocyanate (CTB-FITC) (5μl of 0.2%, Sigma, Saint Louis, MO, USA) were injected intravitreally using 5-μl Hamilton syringe (Hamilton, Bonaduz, Switzerland) [21 (link)]. Briefly, the needle was inserted in peripheral retina, just behind the ora serrata, and was carefully angled and located to avoid unexpected damage. Rats with cataract, retinal detachment, or vitreous hemorrhage were excluded from this study.

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Huang Z., Hu Z., Xie P, & Liu Q. (2017). Tyrosine-mutated AAV2-mediated shRNA silencing of PTEN promotes axon regeneration of adult optic nerve. PLoS ONE, 12(3), e0174096.

Publication 2017

CTB-FITC

Cataract Cholera toxin Fluorescein Isothiocyanate Needle Ora serrata Rats Retina Retinal detachment Subunit Syringe Vectors Vitreous hemorrhage

Corresponding Organization : Jiangsu Province Hospital

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Variable analysis

independent variables

AAV vectors (5μl, titers at 1.0 x 10^12)
Cholera toxin β subunit conjugated fluorescein isothiocyanate (CTB-FITC) (5μl of 0.2%, Sigma, Saint Louis, MO, USA)

dependent variables

Not explicitly mentioned

control variables

Injection technique (needle insertion in peripheral retina, just behind the ora serrata, and carefully angled and located to avoid unexpected damage)
Exclusion of rats with cataract, retinal detachment, or vitreous hemorrhage

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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