Glucosinolate Profiling by HPLC

Freeze-dried samples (100 mg) were boiled in 5 mL water for 10 min. The supernatant was collected after centrifugation, and applied to a DEAE-Sephadex A-25 column. The glucosinolates were converted into their desulpho analogues by overnight treatment with 100 μL of 0.1% aryl sulphatase, and the desulphoglucosinolates were eluted with 1 mL water. HPLC analysis of desulphoglucosinolates was carried out using an Agilent 1260 HPLC instrument equipped with a VWD detector. Samples were separated at 30 °C on a Waters Spherisorb C18 column (250 × 4.6 mm) using acetonitrile and water at a flow rate of 1.0 mL min⁻¹. Absorbance was detected at 226 nm.^{1 (link)}