SAHA Drug Delivery Protocol

Suberoylanilide hydroxamic acid (SAHA) was obtained from Selleck Chemical (Houston, TX, USA). SAHA was dissolved in DMSO at 50 mg/ml to be delivered in a volume that would bypass the potential detrimental effects of excessive DMSO, as described previously.^{40 (link)} Kanamycin (Sigma, St. Louis, MO, USA) was dissolved in 0.9% saline (Hospira, Lake Forest, IL, USA) and delivered by subcutaneous injection. Furosemide (Hospira) was administered by intraperitoneal injection. Tamoxifen (Sigma) was dissolved in corn oil at 25 mg/ml and delivered by intraperitoneal injection at 0.25 mg/g.

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Layman W.S., Williams D.M., Dearman J.A., Sauceda M.A, & Zuo J. (2015). Histone deacetylase inhibition protects hearing against acute ototoxicity by activating the Nf-κB pathway. Cell Death Discovery, 1, 15012-.

Publication 2015

Suberoylanilide hydroxamic acid (SAHA) Kanamycin Furosemide Tamoxifen

0 9 saline Corn oil Dmso Forest Furosemide Intraperitoneal injection Kanamycin Subcutaneous injection Suberoylanilide hydroxamic acid Tamoxifen

Corresponding Organization :

Other organizations : St. Jude Children's Research Hospital

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Variable analysis

independent variables

Suberoylanilide hydroxamic acid (SAHA) concentration
Kanamycin administration
Furosemide administration
Tamoxifen concentration

dependent variables

Not explicitly mentioned

control variables

DMSO volume to bypass potential detrimental effects
Saline solution for Kanamycin administration
Corn oil for Tamoxifen administration

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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