Osmotic minipump installation was conducted according to our previous studies [6 (link), 7 (link), 13 (link)]. An osmotic minipump (No. 2002, 14 days of active life, Alza Corp) was filled with aliskiren dissolved in deionized water (Novartis Pharmaceuticals Co., NJ, USA), which was implanted subcutaneously in the rats under brief anesthesia with sodium pentobarbital (40 mg/kg BW, intraperitoneal injection).
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