Intracellular Nrf2 Nucleoprotein Quantification
Corresponding Organization :
Other organizations : Tianjin Nankai Hospital, Tianjin Medical University
Variable analysis
- None explicitly mentioned
- Intracellular distribution of Nrf2 nucleoprotein
- Kidney tissue samples
- Paraffin embedding
- 4μm sectioning
- Dewaxing in xylene and rehydration with graded ethanol solutions
- Protein blockade
- Incubation with polyclonal Nrf2 primary antibody conjugated to FITC (dilution 1:150)
- Washing with phosphate-buffered saline (PBS)
- Counterstaining with DAPI to visualize nuclei
- Capturing images using a fluorescent microscope (Olympus U-25ND25, Tokyo, Japan) coupled to a digital camera
- Scoring based on the percentage of positive protein in five fields of each slice at 400x magnification using Image-pro plus software
- None explicitly mentioned
- None explicitly mentioned
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