A. baumannii cultures were incubated overnight in brain heart broth (Merck, Darmstadt, Germany) under agitation at 120 rpm at 37 °C. Cell densities were at the optical density: 600 nm (OD600) = 0.8. Subsequently, they were inactivated using 3% formaldehyde (Alphatec®, São Paulo, Brazil) for 2 h at 30 °C, followed by 16 h at 60 °C, under agitation at 120 rpm. Inactivation was confirmed by adding 1 mL of the inactivated culture to Mueller–Hinton agar (Kasvi®, Paraná, Brazil) and incubating at 37 °C for 48 h [32 (link)].
Corresponding Organization : Universidade Federal do Tocantins
Other organizations :
Fundação Oswaldo Cruz, Universidade Federal do Amazonas, Universidade de São Paulo, Universidade Federal de Minas Gerais, Universidade Estadual de Montes Claros, Universidade Federal da Integração Latino-Americana, Universidade Federal de Viçosa
Formaldehyde treatment duration (2 h at 30 °C and 16 h at 60 °C)
dependent variables
Cell density (OD600 = 0.8)
Inactivation confirmation (no growth on Mueller-Hinton agar after 48 h)
control variables
Culture medium (brain heart broth)
Agar medium (Mueller-Hinton agar)
controls
Positive control: Not specified.
Negative control: Not specified.
Annotations
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