Immunohistochemical Analysis of Brain

The brain was fixed in phosphate-buffered 4 % paraformaldehyde. Histological sections were stained with hematoxylin and eosin (HE) by using a standard procedure or immunostained by using a peroxidase-based indirect staining method [30 (link)]. An anti-GFP rabbit polyclonal antibody, an anti-CD31 rabbit polyclonal antibody, an anti-MMP-2 rabbit polyclonal antibody, or an anti-MMP-9 rabbit polyclonal antibody (all from Abcam, Cambridge, United Kingdom) was used as primary antibody. Diaminobenzidine was used as chromogen, and hematoxylin was used for counterstaining.

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Simonsen T.G., Gaustad J.V, & Rofstad E.K. (2015). Intertumor heterogeneity in vascularity and invasiveness of artificial melanoma brain metastases. Journal of Experimental & Clinical Cancer Research : CR, 34, 150.

Publication 2015

anti-GFP rabbit polyclonal antibody anti-CD31 rabbit polyclonal antibody

Anti antibody Antibody Brain Chromogen Eosin Hematoxylin Mmp 2 Mmp 9 Paraformaldehyde Peroxidase Phosphate Rabbit Staining method

Corresponding Organization : Oslo University Hospital

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Variable analysis

independent variables

Immunostaining method (peroxidase-based indirect staining)
Primary antibodies (anti-GFP, anti-CD31, anti-MMP-2, anti-MMP-9)

dependent variables

Staining patterns/expression of GFP, CD31, MMP-2, and MMP-9

control variables

Brain tissue fixed in phosphate-buffered 4% paraformaldehyde
Histological sections stained with hematoxylin and eosin (HE) using a standard procedure

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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