As previously described37 (link), HuMEC-1 were partially activated with 100 μg/ml LPS (Sigma Chemical Co. St. Louis, USA) in MCDB-131 (Life Technologies, Darmstadt, Germany), whereas the other fraction of HuMEC-1 remained non-activated. The treatment of every study group was performed for 60 min under different temperature conditions (Fig. 1A–C). After treatment, medium was removed and transmigration-assay was started.
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