For the kinase assays, the purified Gh-LYK1-ID or Gh-LYK2-ID protein was individually incubated with γ-32P ATP at 30°C for 30 min, as previously described (Liu et al., 2011 (link)). The phosphorylated substrate was visualized with a Typhoon 9200 imager (GE Healthcare) after separation through SDS-PAGE.
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