Multiplex Cytokine Profiling in Patient Sera

Cytokine concentrations in patient sera were quantified by multiplex protein arrays according to the manufacturer’s instructions (BioRad Laboratories, USA) as described recently^{32 (link)}. In brief, 27-Plex and Bio-Plex Pro Human Cytokine 27-Plex (BioRad Laboratories) combined with HGF were used to detect IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8/CXCL8, IL-9, IL-10, IL12(p70), IL-13, IL-15, IL-17, Eotaxin/CCL11, FGF-β, G-CSF, GM-CSF, IFN-γ, IP-10/CXCL10, MCP-1/CCL2, MIP-1a/CCL3, PDGF, MIP-1b/CCL4, RANTES/CCL5, TNF-α, VEGF, and HGF.

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Diestelhorst J., Junge N., Jonigk D., Schlue J., Falk C.S., Manns M.P., Baumann U., Jaeckel E, & Taubert R. (2018). Baseline IL-2 and the AIH score can predict the response to standard therapy in paediatric autoimmune hepatitis. Scientific Reports, 8, 419.

Publication 2018

multiplex protein arrays Bio-Plex Pro Human Cytokine 27-Plex

Ccl11 Ccl2 Ccl3 Ccl4 Ccl5 Cxcl8 Cytokine G csf Gm csf Human Ifn γ Il 10 Il 13 Il 15 Il 17 Il 1ra Il 1β Il12 Patient Pdgf Protein arrays Sera Tnf α Vegf

Corresponding Organization :

Other organizations : Medizinische Hochschule Hannover

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Variable analysis

independent variables

Cytokine concentrations in patient sera

dependent variables

IL-1β
IL-1RA
IL-8/CXCL8
IL-10
IL12(p70)
IL-13
IL-15
IL-17
Eotaxin/CCL11
FGF-β
G-CSF
GM-CSF
IFN-γ
IP-10/CXCL10
MCP-1/CCL2
MIP-1a/CCL3
MIP-1b/CCL4
RANTES/CCL5
TNF-α

control variables

Manufacturer's instructions (BioRad Laboratories, USA) as described recently^{32 (link)}
27-Plex and Bio-Plex Pro Human Cytokine 27-Plex (BioRad Laboratories)

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