E. coli strain DH5α was used for plasmid construction while SHuffle T7 Express (New England Biolabs)20 (link) was used for cyclonal expression and library selections. Protein antigens for immunoassays including GST-Gcn4-PP, GST-HAG, GST-c-Myc, and GST-c-Jun were expressed using E. coli T7 Express (New England Biolabs).
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