Blood pressure was monitored using a cannula placed in the femoral artery as previously described29 (link). Briefly, a heparinized polyethylene cannula was inserted 3 cm proximally into the left femoral artery and secured to the surrounding tissue. The line was connected to a pressure transducer (Transpac, Abbott Critical Care Systems, Sligo, Ireland), whose signal (Bridge Amp ML 110, Amplifier ML 785, Powerlab/8SP, ADInstruments, Colorado Springs, CO, USA) gave direct and continuous BP monitoring (Lab Chart 7, ADInstruments).
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