Protein extracts for Western blot analysis were obtained as described previously [17 (link)]. For Western blot detection, we used NUMB (ab4147, Abcam, 1 μg/mL) and NUMBL (ab37500, Abcam, 1 μg/mL) antibodies. α-Tubulin (T9026, Sigma) was used as a control. Horseradish peroxidase-labeled rabbit anti-mouse (ab97046, Abcam, diluted 1:5,000) and goat anti-rabbit (ab97051, Abcam, diluted 1:5,000) secondary antibodies were used.
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