3T3-L1 Fibroblast Differentiation Protocol

3T3-L1 fibroblasts culture and differentiation were previously described in (Liu and Pilch, 2008 (link)). PTRF null and control wild type primary MEFs cells used in all experiments were maintained within 3–5 passages. When they reached 90% confluence, they were transfected with the cDNA of interest by means of the X-tremeGENE HP reagent (Roche, Indianapolis, IN). HEK293 cells were cultured and transfected as describe in (Liu and Pilch, 2008 (link)).

Free full text: Click here

Liu L, & Pilch P.F. (2016). PTRF/Cavin-1 promotes efficient ribosomal RNA transcription in response to metabolic challenges. eLife, 5, e17508.

Publication 2016

X-tremeGENE HP reagent

3t3 l1 Cdna Cells Fibroblasts Hek293 cells

Corresponding Organization :

Other organizations : Boston University

Top 5 similar protocols

Variable analysis

independent variables

Genetic manipulation (PTRF null vs. control wild type primary MEFs cells)

dependent variables

Not explicitly mentioned

control variables

Passage number (3-5 passages) for PTRF null and control wild type primary MEFs cells
Cell confluence (90% confluence) for transfection
Transfection method (X-tremeGENE HP reagent)

controls

Positive control: HEK293 cells cultured and transfected as described in (Liu and Pilch, 2008)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!