Cytokine and Mediator ELISA Analysis

The concentration of IL-6, TNF-α, iNOS, MCP-1, IL-10, Arg-1, Fizz-1, and TGF-β in the culture supernatants was determined with commercial enzyme-linked immunosorbent assay (ELISA) kits following the manuals. Human IL-6 (ab178013), TNF-alpha (ab285312), iNOS (ab253217), MCP-1 (ab179886), IL-10 (ab185986), TGF beta 1 (ab100647) ELISA kits were provided by Abcam. Arginase-1 Human ELISA Kit (BMS2216TEN) and Human Fizz-1 ELISA Kit (SEKH-0305) were obtained from Thermo Fisher Scientific and SOLARBIO (Beijing, China), respectively.

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Shen H.Y., Xu J.L., Zhang W., Chen Q.N., Zhu Z, & Mao Y. (2024). Exosomal circRHCG promotes breast cancer metastasis via facilitating M2 polarization through TFEB ubiquitination and degradation. NPJ Precision Oncology, 8, 22.

Publication 2024

Arginase-1 Human ELISA Kit

Corresponding Organization : Second Affiliated Hospital of Nanjing Medical University

Other organizations : Nanjing Medical University, Jiangsu Province Hospital, Second People’s Hospital of Huai’an, Xuzhou Medical College

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Concentration of IL-6
Concentration of TNF-α
Concentration of iNOS
Concentration of MCP-1
Concentration of IL-10
Concentration of Arg-1
Concentration of Fizz-1
Concentration of TGF-β

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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