Evaluating MRP Transporter Inhibitors in Cell Lines

MDCKII-WT, MDCKII-MRP1 and MDCKII-MRP4 cells were obtained from Netherlands Cancer Institute (Dr. A. H. Schinkel), Amsterdam, Netherlands. Hep G2 cell was purchased from ATCC (Manassas, VA, USA). hCMEC/D3 cells were obtained from Dr. Pierre-Olivier Couraud (Institute Cochin, Paris, France). Cell culture medium and fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific (Grand Island, NY, USA). The non-selective MRP inhibitor MK 571 was from Cayman Chemical (Ann Arbor, MI, USA) and selective MRP1 inhibitor reversan[33 (link), 34 (link)] and selective MRP4 inhibitor ceefourin 1[35 (link), 36 (link)] were from Sigma (St. Louis, MO, USA) and ABCAM (Cambridge, MA, USA). [³H]Benzylpenicillin (25 Ci/mmol) and [¹⁴C]mannitol (55 mCi/mmol) were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA).