Protocol detail

Assessing TLR-Induced Inflammatory Responses

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Paired strains from a prototypical case^{5 (link)} were challenged with stimuli that activate the full range of human TLRs using the Human TLR1-9 Agonist Kit (InvivoGen USA, San Diego, CA). IL-6 was analyzed by ELISA^{4 , 7}; IL-6 is a measure of TLR pro-inflammatory activity^{8 (link), 9 (link), 12 –15 (link)}.

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Falsetta M.L., Foster D.C., Woeller C.F., Pollock S.J., Bonham A.D., Piekna-Przybylska D., Maggirwar S.B., Haidaris C.G, & Phipps R.P. (2018). Toll-like receptor signaling contributes to proinflammatory mediator production in localized provoked vulvodynia. Journal of lower genital tract disease, 22(1), 52-57.

Publication 2017

Human TLR1-9 Agonist Kit

Human Inflammatory Strains

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Variable analysis

independent variables

Paired strains from a prototypical case
Stimuli that activate the full range of human TLRs using the Human TLR1-9 Agonist Kit

dependent variables

IL-6 levels (measured by ELISA)

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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