Confocal and AiryScan Microscopy Protocols

Confocal and AiryScan imaging were performed using LSM880, LSM800 (Carl Zeiss Microscopy) microscopes with 63X/1.40 NA plan-apochromat oil immersion objective and 32-channel gallium arsenide phosphide (GaAsP)-photomultiplier tubes (PMT) area detector. For CLEM, light microscopy was performed with an Andor DragonFly microscope with either a PlanApo 63X/1.4 NA oil immersion objective or a 20X air objective and equipped with a Zyla cMOS camera. 405nm, 488 nm, 561 nm and 633 laser lines were used.

Free full text: Click here

Falahati H., Wu Y, & De Camilli P. (2024). Ectopic Reconstitution of a Spine-Apparatus Like Structure Provides Insight into Mechanisms Underlying Its Formation. bioRxiv.

Publication Preprint 2024

LSM880 LSM800

Corresponding Organization : Howard Hughes Medical Institute

Top 5 similar protocols

Variable analysis

independent variables

Confocal and AiryScan imaging
Light microscopy

dependent variables

Measured outcomes

control variables

LSM880, LSM800 (Carl Zeiss Microscopy) microscopes
63X/1.40 NA plan-apochromat oil immersion objective
32-channel gallium arsenide phosphide (GaAsP)-photomultiplier tubes (PMT) area detector
Andor DragonFly microscope with PlanApo 63X/1.4 NA oil immersion objective or 20X air objective
Zyla cMOS camera
405nm, 488 nm, 561 nm and 633 laser lines

controls

No explicit positive or negative controls mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!