Descriptions of the plasmid sources and null mutant cells used in the present study are listed in Supplementary Table S1 . Cells were transformed with expression vectors of GFP-tagged proteins were transformed into cells by electroporation or laserporation, as described previously [46 (link),47 (link)]. The transformed cells were selected in HL5 medium containing 10 µg/mL G418 (Wako Pure Chemical Corporation, Osaka, Japan) or 10 µg/mL blasticidin S hydrochloride (Wako Pure Chemical Corporation, Osaka, Japan) in plastic dishes.
We constructed expression vectors for some GFP-tagged proteins, including RacA, severin, filamin, ABD34, CAP32, fimbrin, α-actinin, and CARMIL. The individual genes were amplified from a cDNA library by PCR and subcloned between the BamH1 and Sac1 sites downstream of the C-terminal GFP site in the pA15GFP expression vector, as described previously [16 (link)].
We constructed expression vectors for some GFP-tagged proteins, including RacA, severin, filamin, ABD34, CAP32, fimbrin, α-actinin, and CARMIL. The individual genes were amplified from a cDNA library by PCR and subcloned between the BamH1 and Sac1 sites downstream of the C-terminal GFP site in the pA15GFP expression vector, as described previously [16 (link)].
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