Investigating Tumor Growth Regulation by MGL

Parental KP cells (0.5×10⁶) were injected (s.c.) into the right flank of mice. For pharmacological inhibition of MGL, tumor-bearing C57BL/6J wild-type mice were intraperitoneally (i.p.) treated with 16 mg/kg JZL184 (MGL inhibitor, Cayman), once daily, starting one day prior to the injection of tumor cells. For the experiments with MGL-overexpressing cells, either 0.5 × 10⁶ KP _MGL or KP _ctrl cells were injected (s.c.) into the right flank of C57BL/6J wild-type mice. Tumor growth was monitored during the course of the experiments twice per week using a caliper. Mice were sacrificed after about two weeks, and tumors were subsequently collected, weighted, and measured with a caliper ex vivo. Tumor volume was calculated according to the following formula: v = length x width x height x π/6.^{40 (link)}