Antibody Validation for Stem Cell Markers

The following antibodies were used for western blotting: rabbit monoclonal anti-CD24 (1:500, catalog number ab179821, Abcam, MA, USA), rabbit monoclonal anti-CD44 (1:500, catalog number ab189524, Abcam), rabbit recombinant multiclonal anti-CD133 (1:800, catalog number ab278053, Abcam), mouse monoclonal anti-Nanog (1:1000, catalog number ab173368, Abcam), rabbit monoclonal anti-OCT4 (1:500, catalog number ab200834, Abcam), mouse monoclonal anti-SOX2 (1:1000, catalog number ab79351, Abcam), rabbit polyclonal anti-DLG5 (1:1000, catalog number ab231283, Abcam), rabbit polyclonal anti-PRDM16 (1:1000, catalog number ab106410, Abcam), mouse monoclonal anti-ErbB-2 (1:500, catalog number sc-33684, Santa Cruz Biotechnology, CA, USA), and mouse monoclonal anti-β-actin (1:1000, catalog number A5441, Sigma-Aldrich, MO, USA). Other primary antibodies and secondary antibodies and experimental procedures for immunoblotting are provided in the supplementary experimental procedures^{10 (link)}.

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Bai W., Peng H., Zhang J., Zhao Y., Li Z., Feng X., Zhang J., Liang F., Wang L., Zhang N., Li Y., Zhu H, & Ji Q. (2022). LINC00589-dominated ceRNA networks regulate multiple chemoresistance and cancer stem cell-like properties in HER2+ breast cancer. NPJ Breast Cancer, 8, 115.

Publication 2022

ab179821 ab189524 ab278053 ab200834 ab79351 ab231283 ab106410 sc-33684 A5441

Actin Anti erbb 2 Antibodies Cd44 Mouse Oct4 Prdm16 Rabbit Sox2

Corresponding Organization : Xijing Hospital

Other organizations : Urumqi General Hospital of PLA, Chinese People's Liberation Army

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Variable analysis

independent variables

Antibodies used for western blotting

dependent variables

Protein expression levels of CD24, CD44, CD133, Nanog, OCT4, SOX2, DLG5, PRDM16, and ErbB-2

control variables

Antibody dilution ratios
β-actin as a loading control

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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