Immunohistochemical Analysis of LIGHT

The specimens were sliced into 4 μm sections, covered with paraffin, and stored at 4°C until immunohistochemistry was performed. The immunohistochemistry procedure was based on our previous study (15 (link)) with slight modifications. The immunohistochemistry kit was obtained from Zhongshan Golden Bridge Biotechnology, Beijing, Co., Ltd., and included 3% H2O2, normal goat serum for blocking streptavidin marked with horseradish peroxidase, and diaminobenzidine reaction agent. The sodium citrate antigen retrieval solution was acquired from Beijing Solarbio Science & Technology Co., Ltd. The polyclonal antibody against LIGHT was acquired from Sigma-Aldrich (HPA012700) and was applied at a concentration of 1:50.

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Han M., Sun Y., Zhao W., Xiang G., Wang X., Jiang Z., Xue Z, & Zhou W. (2022). Comprehensive characterization of TNFSF14/LIGHT with implications in prognosis and immunotherapy of human gliomas. Frontiers in Immunology, 13, 1025286.

Publication 2022

immunohistochemistry kit sodium citrate antigen retrieval solution

Antibody Antigen Goat H2o2 Horseradish peroxidase Immunohistochemistry Light Normal Paraffin Serum Sodium citrate Streptavidin

Corresponding Organization : Qilu Hospital of Shandong University

Other organizations : Shandong University

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Variable analysis

independent variables

Slicing the specimens into 4 μm sections
Covering the sections with paraffin
Storing the sections at 4°C
Performing immunohistochemistry with slight modifications to the previous study

dependent variables

Immunohistochemistry outcomes

control variables

Concentration of the polyclonal antibody against LIGHT (1:50)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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