The instrumental analysis was performed by a Waters ACQUITY UPLC system (Waters, Millford, MA, USA) composed of a binary solvent manager, sample manager and photo diode array detector (PDA). The chromatographic separation was accomplished on a ACQUITY BEH C18 column (100 mm×2.1 mm, 1.7 μm; Waters). The column temperature was 40℃. The binary gradient elution system consisted of 0.001% phosphoric acid in water (A) and 0.001% phosphoric acid in acetonitrile (B). The separation was achieved using the following gradient program: 0-0.5 min (15% B), 14.5 min (30% B), 15.5 min (32% B), 18.5 min (38% B), 24.0 min (43% B), 27.0 min (55% B), 27.0-31.0 min (55% B), 35.0 min (70% B), 38.0 min (90% B), 38.1 min (15% B), and 38.1-43.0 min (15% B). The flow rate was set at 0.6 mL/min and the sample injection volume was 2.0 μL. The 30 ginsenosides were detected by PDA at 203 nm.