Prepare matrigel and add it to the lower hole of the ibidi angiogenesis slide. Put the matrigel glue into the incubator to make it coagulate. Prepare a cell suspension with a density of 2 × 10 5 cells/mL, and add 50 μL of the cell suspension to the solidified ibidi angiogenesis slide. According to the growth rate of the cells, images are collected periodically for observation.
Protocol detail
Angiogenesis Assay with Matrigel
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Corresponding Organization :
Other organizations : Affiliated Hospital of Nantong University, Nantong University, Xuzhou Central Hospital
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Variable analysis
independent variables
- Matrigel preparation and addition to the lower hole of the ibidi angiogenesis slide
- Cell suspension density (2 × 10^5 cells/mL)
- Volume of cell suspension added (50 μL)
- Cellular growth and angiogenesis over time
- Incubation time for Matrigel coagulation
- Ibidi angiogenesis slide
- No explicit controls mentioned
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