Nopalea cochenillifera cv. Maya (edible Opuntia sp., common name “Kasugai Saboten”; subfamily Opuntioideae, family Cactaceae) was bought at a local market (Goto Cactus, 1-122-3, Momoyama, Kasugai City, Aichi Prefecture, Japan). The cladode was dried for 10 days in the dark at room temperature and then placed in a 1 dm−3 pot filled with a mixture of field soil and leaf mold (7:3, v/v) for about 3 weeks until rooting in a glass greenhouse (Meijo University Tempaku Campus, Tempaku, Nagoya City, Aichi Prefecture, Japan). The greenhouse was warmed by boilers at ≥ 10 °C from November to March.
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