Blood DNA Extraction and Genotyping

DNA was extracted from 2uL of whole blood using the DNA Extract All Reagents Kit (ThermoFisher). Genotyping of rs678849 was performed on an ABI 7900 Thermocycler using a Taqman SNP Genotyping Assay (ThermoFisher) as previously described ^{34 (link)}.

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Crist* R.C., Phillips K.A., Furnari M.A., Moran L.M., Doyle G.A., McNicholas L.F., Cornish J.W., Kampman K.M., Preston K.L, & Berrettini W.H. (2018). Replication of the pharmacogenetic effect of rs678849 on buprenorphine efficacy in African-Americans with opioid use disorder. The pharmacogenomics journal, 19(3), 260-268.

Publication 2018

DNA Extract All Reagents Kit Taqman SNP Genotyping Assay

Assay Blood

Corresponding Organization : University of Pennsylvania

Other organizations : National Institute on Drug Abuse, Philadelphia VA Medical Center

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Variable analysis

independent variables

Genotyping of rs678849

dependent variables

Genotyping of rs678849

control variables

Amount of whole blood used (2uL)
DNA extraction method (DNA Extract All Reagents Kit, ThermoFisher)
Genotyping platform (ABI 7900 Thermocycler)
Genotyping assay (Taqman SNP Genotyping Assay, ThermoFisher)

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