Visualization of Extracellular Vesicles
Corresponding Organization : Istituti di Ricovero e Cura a Carattere Scientifico
Other organizations : University of Urbino, Ospedale G.B. Morgagni - L.Pierantoni, Ospedale “M. Bufalini” di Cesena, University of Bologna
Variable analysis
- Adsorption of EVs isolated from serum and urinary supernatant to formvar carbon-coated 200 mesh grids for 20 min
- Incubation of grids with 2% (w/v) sodium phosphotungstate for 1 min
- Observation of specimens by means of a Philips CM10 transmission electron microscope at 80 kV
- Formvar carbon-coated 200 mesh grids
- Removal of liquid excess with filter paper after negative staining
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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