Log In Sign up
The largest database of trusted experimental protocols
Protocol detail

Culturing Primary Tumor Organoids

Protocol full text hidden due to copyright restrictions

However, the full text is available at the following link: Access Free Full Text.

Boj S.F., Hwang C.I., Baker L.A., Chio I.I., Engle D.D., Corbo V., Jager M., Ponz-Sarvise M., Tiriac H., Spector M.S., Gracanin A., Oni T., Yu K.H., van Boxtel R., Huch M., Rivera K.D., Wilson J.P., Feigin M.E., Öhlund D., Handly-Santana A., Ardito-Abraham C.M., Ludwig M., Elyada E., Alagesan B., Biffi G., Yordanov G.N., Delcuze B., Creighton B., Wright K., Park Y., Morsink F.H., Molenaar I.Q., Borel Rinkes I.H., Cuppen E., Hao Y., Jin Y., Nijman I.J., Iacobuzio-Donahue C., Leach S.D., Pappin D.J., Hammell M., Klimstra D.S., Basturk O., Hruban R.H., Offerhaus G.J., Vries R.G., Clevers H, & Tuveson D.A. (2014). Organoid Models of Human and Mouse Ductal Pancreatic Cancer. Cell, 160(0), 324-338.
Publication 2014
collagenase II TrypLE HEPES Glutamax penicillin/streptomycin Primocin N-acetyl-L-cysteine Gastrin Nicotinamide A83-01 soybean trypsin inhibitor
A83 01 Cells Collagenase Conditioned medium Cultured medium Digestion Epidermal growth factor Fgf10 Fibroblast growth factor 10 Gastrin Hepes Human Matrigel N acetyl l cysteine Nicotinamide Noggin Penicillin Pge2 Recombinant protein Soybean Streptomycin Tissue Trypsin inhibitor Tumor

Corresponding Organization : Memorial Sloan Kettering Cancer Center

Other organizations : Hubrecht Institute for Developmental Biology and Stem Cell Research, Stony Brook University, Cornell University, University Medical Center Utrecht, Johns Hopkins Medicine, Johns Hopkins University, Cancer Research Center

Protocol comparator
Top 5 similar protocols

Sign up for free or view similar protocols

Protocol cited in 119 other protocols

Variable analysis

Dependent Variables detection not available due to copyright restrictions.
Unable to provide accurate variables.

Annotations

Annotations not available due to copyright restrictions

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!