Immunofluorescence using Gpp130 and Golgin97

Immunofluorescence was carried out as described previously (Lavieu et al., 2014 (link)). Anti-Gpp130 and anti-Golgin97 antibodies were purchased from Covance and Abcam, respectively. Anti-rabbit Atto594 (Rockland), anti-mouse Atto594 (Sigma-Aldrich), and anti-mouse- and anti-rabbit 635P (Abberior) secondary antibodies were used for the STED experiments.

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Bottanelli F., Kilian N., Ernst A.M., Rivera-Molina F., Schroeder L.K., Kromann E.B., Lessard M.D., Erdmann R.S., Schepartz A., Baddeley D., Bewersdorf J., Toomre D, & Rothman J.E. (2017). A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi. Molecular Biology of the Cell, 28(12), 1676-1687.

Publication 2017

Anti-Gpp130 anti-mouse Atto594

Anti antibodies Antibodies Golgin97 Immunofluorescence Mouse Rabbit

Corresponding Organization :

Other organizations : Yale University

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Variable analysis

independent variables

Anti-Gpp130 antibody
Anti-Golgin97 antibody

dependent variables

Immunofluorescence signal

control variables

Experimental protocol (Lavieu et al., 2014)
Anti-rabbit Atto594 secondary antibody
Anti-mouse Atto594 secondary antibody
Anti-mouse- and anti-rabbit 635P secondary antibodies

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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