Immunostaining and Western Blot Antibodies

Antibodies used for immunostaining: rabbit- anti Olig2 (EMD Millipore AB9610, 1:500), mouse-anti APC [CC1] (EMD Millipore OP80, 1:500), mouse-anti Ki67 (BD Pharmingen 550609, 1:50), donkey anti-rabbit IgG (H + L) secondary- Alexa Fluor 568 (Thermo Fisher Scientific A10042, 1:300) and donkey anti-mouse IgG (H + L) secondary- Alexa Fluor 488 (Thermo Fisher Scientific A21202, 1:300). These antibodies have been documented in a previous porcine study (Ishibashi et al., 2012 (link)).
Antibodies used for western blots: rabbit-anti MBP (Cell signaling 78896, 1:1,000), rabbit-anti PLP1 (Cell Signaling Technology 85971S, 1:1,000), rabbit-anti HMGCS1 (Cell Signaling Technology 42201S, 1:1,000), rabbit-anti GAPDH (Cell Signaling Technology 5174, 1:1,000), mouse anti-β-Actin C4 (Santa Cruz 47778, 1:1,000), mouse anti-MAG (Santa Cruz 166849, 1:1,000), goat anti-MOG (Novus Biologicals NB300–948, 1:1,000), goat anti-rabbit IgG (H+L)-HRP (Thermo Fisher Scientific G21234, 1:5,000), goat anti-mouse IgG (H+L)-HRP (Thermo Fisher Scientific G16072, 1:5,000), donkey anti-goat IgG (H+L)-HRP (Thermo Fisher Scientific A15999, 1:5,000). Gray matter tissue was used as a negative control.