To reconstruct the evolutionary history of the p53 domain in animals, the selection of TP53 homologues covering the diversity of the family is necessary.
In this example, the p53 homologues of diverse animals (the cnidarian Hydra vulgaris, four insect species: Drosophila melanogaster, Apis mellifera, Bombus terrestris and Aedes aegyptus, and the tunicate Ciona intestinalis) and the p53, p63, and p73 of diverse vertebrates (the teleost fish Danio rerio, the coelacanth Latimeria chalumnae, the amphibian Xenopus tropicalis, the lizard Anolis carolinensis, the bird Gallus gallus, and the mammals Bos taurus and H. sapiens) were chosen. The p53 of the choanoflagellate Monosiga brevicollis (a protist related to animals), also retrieved from a BLAST search was chosen as outgroup( S1 File ).
In this example, the p53 homologues of diverse animals (the cnidarian Hydra vulgaris, four insect species: Drosophila melanogaster, Apis mellifera, Bombus terrestris and Aedes aegyptus, and the tunicate Ciona intestinalis) and the p53, p63, and p73 of diverse vertebrates (the teleost fish Danio rerio, the coelacanth Latimeria chalumnae, the amphibian Xenopus tropicalis, the lizard Anolis carolinensis, the bird Gallus gallus, and the mammals Bos taurus and H. sapiens) were chosen. The p53 of the choanoflagellate Monosiga brevicollis (a protist related to animals), also retrieved from a BLAST search was chosen as outgroup
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