A human lamin A cDNA was subcloned into the pTracer-CMV vector (Invitrogen Corp.). The linearized vector was transfected into lamin A/C −/− mouse embryonic fibroblasts (MEFs). Stable clones were selected using Zeocin, according to the manufacturer's instructions and the clones pooled. Subsequent analysis showed that the cells in the pool were heterogeneous with regard to lamin A expression.
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Lamin A Complementation in MEFs
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Other organizations : BioScience Laboratories (United States), National Cancer Institute, Science Applications International Corporation (United States), University of Calgary
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Variable analysis
independent variables
- Transfection of linearized pTracer-CMV vector containing human lamin A cDNA into lamin A/C −/− mouse embryonic fibroblasts (MEFs)
- Lamin A expression in the stable clones
- Selection of stable clones using Zeocin according to the manufacturer's instructions
- Negative control: Lamin A/C −/− mouse embryonic fibroblasts (MEFs) without transfection
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