Flow cytometry analysis was performed using NovoCyte 2060R (ACEA Biosciences, San Diego, CA, USA) according to previous report (17 (link)). The transduction efficiency of IM19 CAR gene into T cells was detected using a phycoerythrin (PE)-labeled anti-CAR antibody which was developed by Immunochina Pharmaceuticals for recognizing the scFv of IM19. PE-anti-CD4 and FITC-anti-CD8 antibodies (BD Biosciences, San Jose, CA, USA) were used to detect the CD4/CD8 ratio. The APC-anti-CD45RA and FITC-anti-CD62L antibodies (Biolegend, San Diego, CA, USA) were used to evaluate the ratio of memory T cells.
Protocol detail
Flow Cytometry Analysis of CAR-T Cells
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Anti antibodies
Anti antibody
Cd4 cd8 ratio
Cd62l
Fitc
Flow cytometry
Gene transduction
Memory t cells
Pharmaceuticals
Phycoerythrin
T cells
Corresponding Organization :
Other organizations : Peking University, Peking University Cancer Hospital, Shandong First Medical University, Shandong Tumor Hospital
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Variable analysis
independent variables
- The transduction efficiency of IM19 CAR gene into T cells
- The transduction efficiency of IM19 CAR gene into T cells (detected using a phycoerythrin (PE)-labeled anti-CAR antibody)
- The CD4/CD8 ratio (detected using PE-anti-CD4 and FITC-anti-CD8 antibodies)
- The ratio of memory T cells (evaluated using APC-anti-CD45RA and FITC-anti-CD62L antibodies)
- Flow cytometry analysis was performed using NovoCyte 2060R (ACEA Biosciences, San Diego, CA, USA) according to previous report (17 (link)).
- Positive control: Not specified
- Negative control: Not specified
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