Regulating ToElovl5 Elongases via PPARα

RNA interference (siRNA) of PPARαb (PPARαb-si) and corresponding negative controls (si-NC) were purchased from Genecreate (Wuhan, China). Lipofectamine RNAiMAX transfection reagent (Invitrogen, United States) was used for transfection in TOCF cells. The PPARαb siRNA sequence is listed in Supplementary Table S1. Additionally, the agonist and inhibitor of PPARα were used to clarify the role of the transcription factor in the regulation of ToElovl5 elongases. WY-14643 (0.1, 1, and 4 μmol/L, Sigma, United States) was used as a PPARαb agonist, whereas GW6471 (0.1, 1, and 4 μmol/L, Sigma, United States) was used as a PPARαb inhibitor. Total RNA was extracted from TOCF cells as described above. The experiment was performed according to Li et al. (2017) (link).