Culturing Diverse Cell Lines for Research

Cell lines SW480 (Dukes’ type B, colorectal adenocarcinoma; ATCC^® CCL-228^™), HCT-116 (colorectal carcinoma; ATCC^® CCL-247^™), A2780 and A2780cis (ovarian carcinoma, Sigma), KB-3-1 (Human papillomavirus-related endocervical adenocarcinoma; HELA derivative, obtained from Dr. D. W. Shen, Bethesda, Maryland) VM47 (primary melanoma as described previously^{50 (link)}), H35 and H52 (primary glioblastoma as described previously^{51 (link)}) and Capan-1 (liver metastatic pancreatic cancer; ATCC^® HTB-79^™) were grown as a monolayer at 37 °C in a humified 5% CO₂ atmosphere. RPMI growth medium was supplemented with 10% fetal bovine serum (PAA, Linz, Austria). Cell cultures were periodically checked for Mycoplasma contamination. All media were supplemented with 2 mM MES buffer (pH 6, 1% vol) or H₂O (1% vol).

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Alonso-de Castro S., Terenzi A., Hager S., Englinger B., Faraone A., Martínez J.C., Galanski M.S., Keppler B.K., Berger W, & Salassa L. (2018). Biological activity of PtIV prodrugs triggered by riboflavin-mediated bioorthogonal photocatalysis. Scientific Reports, 8, 17198.

Publication 2018

CCL-228 CCL-247 A2780 A2780cis KB-3-1 HTB-79

Adenocarcinoma Atmosphere Buffer Cell cultures Cell lines Colorectal carcinoma Endocervical Fetal bovine serum Glioblastoma Growth medium Hela Human papillomavirus Liver Liver cancer Melanoma Metastatic cancer Mycoplasma Ovarian carcinoma Pancreatic Pancreatic cancer

Corresponding Organization : Ikerbasque

Other organizations : CIC biomaGUNE, Donostia International Physics Center, Comprehensive Cancer Center Vienna, Medical University of Vienna, University of Vienna

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Variable analysis

independent variables

Cell lines: SW480, HCT-116, A2780, A2780cis, KB-3-1, VM47, H35, H52, Capan-1

dependent variables

Not explicitly mentioned

control variables

Cells were grown as a monolayer at 37 °C in a humified 5% CO2 atmosphere
RPMI growth medium was supplemented with 10% fetal bovine serum (PAA, Linz, Austria)
Cell cultures were periodically checked for Mycoplasma contamination
All media were supplemented with 2 mM MES buffer (pH 6, 1% vol) or H2O (1% vol)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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