Phytochemical Profiling of FFBJ and FFOJ

Qualitative and quantitative composition of FFBJ and FFOJ were determined using previously described protocol [21 (link), 22 (link)]. Briefly, both FFBJ and FFOJ were solubilized in methanol to a concentration of 1 mg/mL⁻¹, ultrasonicated and filtered through a 0.2 μm nylon membrane (Millipore, Milan, Italy), and then injected into a UHPLC coupled online to an LCMS-IT-TOF mass spectrometer (Shimadzu, Kyoto, Japan). Identification of flavonoids was carried out on the basis of diode array spectra, MS molecular ions, and MS/MS fragmentation patterns. Data obtained were compared with those available in scientific literature. Molecular formulae were calculated by the Formula Predictor software (Shimadzu).

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Ferlazzo N., Visalli G., Smeriglio A., Cirmi S., Lombardo G.E., Campiglia P., Di Pietro A, & Navarra M. (2015). Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 957031.

Publication 2015

0.2 μm nylon membrane LCMS-IT-TOF mass spectrometer Formula Predictor software

Flavonoids Ions Lcms Membrane Methanol Ms ms Nylon

Corresponding Organization :

Other organizations : University of Messina, University of Salerno

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Variable analysis

independent variables

The composition (qualitative and quantitative) of FFBJ and FFOJ

dependent variables

The identification and quantification of flavonoids in FFBJ and FFOJ

control variables

Solubilization of FFBJ and FFOJ in methanol to a concentration of 1 mg/mL^-1
Ultrasonication of the solutions
Filtration through a 0.2 μm nylon membrane
Injection into a UHPLC coupled online to an LCMS-IT-TOF mass spectrometer

controls

No positive or negative controls were explicitly mentioned in the provided information.

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