Ethanol-Induced Autophagy Modulation

A TM4 (#88111401, ECACC, Salisbury, Wilts, UK) SC line was cultured in a Dulbecco’s Modified Eagle’s medium (DMEM)/F-12 nutrient mixture (D8437, Sigma-Aldrich, St. Louis, MO, USA) containing 5% horse serum (MIC2921149, MP Bio, Tokyo, Japan) and 2.5% fetal bovine serum (FBS) (S1820, Bio West, Nuaille, France) at 37 °C in 5% CO₂. The cells were treated with ethanol (50 or 100 mmol/L,) with and without autophagy inhibitor 3-MA (sc-205596, Santa-Cruz Biotechnology, TX, USA) as reported by others [41 (link),44 (link)].

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Horibe A., Eid N., Ito Y., Hamaoka H., Tanaka Y, & Kondo Y. (2017). Upregulated Autophagy in Sertoli Cells of Ethanol-Treated Rats Is Associated with Induction of Inducible Nitric Oxide Synthase (iNOS), Androgen Receptor Suppression and Germ Cell Apoptosis. International Journal of Molecular Sciences, 18(5), 1061.

Publication 2017

S1820

Autophagy Cells Cultured medium Eagle Ethanol Fetal bovine serum Horse Nutrient Serum

Corresponding Organization : Osaka Medical and Pharmaceutical University

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Variable analysis

independent variables

Ethanol (50 or 100 mmol/L)
Autophagy inhibitor 3-MA (sc-205596, Santa-Cruz Biotechnology, TX, USA)

dependent variables

Not explicitly mentioned

control variables

Culture medium: Dulbecco's Modified Eagle's medium (DMEM)/F-12 nutrient mixture (D8437, Sigma-Aldrich, St. Louis, MO, USA)
Serum: 5% horse serum (MIC2921149, MP Bio, Tokyo, Japan) and 2.5% fetal bovine serum (FBS) (S1820, Bio West, Nuaille, France)
Temperature: 37 °C
Atmosphere: 5% CO2

controls

Positive control: Not mentioned
Negative control: Cells treated without ethanol and 3-MA (as per the references [41, 44])

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