Human Myeloma Cell Line Cultivation

Human myeloma cell lines H929, MM.1r, and RPMI-8226 were purchased from ATCC and cultured at 37°C, 5% CO₂ in RPMI-1640 (Invitrogen, A1049101) supplemented with 10% heat inactivated fetal bovine serum (Sigma-Aldrich, F0926). PCNY1 is a clonal cell line derived from a primary patient sample previously described, which is p53 null (17p-; p53 p. Arg248Gly) [9 (link)]. PCNY1 cells were cultured at 37°C, 5% CO₂ in X-VIVO 15 (Lonza, 04-744Q) supplemented with 10% heat inactivated fetal bovine serum (Sigma-Aldrich, F0926) and 1ng/mL IL-6 (R&D, 206-IL). HEK293T cells were purchased from ATCC and cultured at 37°C, 5% CO₂ in DMEM (Corning, 15-017-CV) supplemented with 10% heat inactivated fetal bovine serum (Sigma-Aldrich, F0926), 1mM sodium pyruvate (Corning, 25-000-CI) and 6 mM L-glutamine (Gibco, 25030-081).