Lentiviral Knockdown of PTK2B in Cancer Cells

Lentiviral PTK2B shRNA plasmids were purchased from Sigma-Aldrich (MISSION shRNA Plasmid DNA protein tyrosine kinase 2 beta (PTK2B/PYK2) SHCLND-NM_004103. 3-763S1C1 & NM_004103. 3-4018S21C1). The lentiviral packaging mix was also purchased from Sigma (SHP001). Plasmids were transfected using Lipofectamine™ 3000 Reagent (L3000001, ThermoFisher Scientific) and following manufacturer recommendations. The lentiviral particles used to infect SkBr3 and MDA-MB-453 were produced corresponding to the manufacturer recommendations and as previously described [29 (link)].

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Al-Juboori S.I., Vadakekolathu J., Idri S., Wagner S., Zafeiris D., Pearson J.R., Almshayakhchi R., Caraglia M., Desiderio V., Miles A.K., Boocock D.J., Ball G.R, & Regad T. (2019). PYK2 promotes HER2-positive breast cancer invasion. Journal of Experimental & Clinical Cancer Research : CR, 38, 210.

Publication 2019

MISSION shRNA SHP001 Lipofectamine™ 3000 Reagent

Lipofectamine Plasmids Ptk2b Shrna

Corresponding Organization :

Other organizations : Nottingham Trent University, University of Campania "Luigi Vanvitelli"

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Variable analysis

independent variables

Lentiviral PTK2B shRNA plasmids

dependent variables

Infection of SkBr3 and MDA-MB-453 cell lines

control variables

Lipofectamine™ 3000 Reagent
Lentiviral packaging mix

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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